2 edition of Gene-based detection of microorganisms in environmental samples using PCR found in the catalog.
Gene-based detection of microorganisms in environmental samples using PCR
by National Aeronautics and Space Administration, National Technical Information Service, distributor in [Washington, D.C, Springfield, Va
Written in English
|Other titles||Gene based detection of microorganisms in environmental samples using PCR|
|Statement||John I. Glass ... [et al.].|
|Series||NASA-TM -- 112923, NASA technical memorandum -- 112923..|
|Contributions||Glass, John I., United States. National Aeronautics and Space Administration.|
|The Physical Object|
Bioaerosol studies aim to describe the microbial content and increase understanding of the aerosolization processes linked to diseases. Air samplers are used to collect, identify, and quantify bioaerosols. Studies comparing the performances of air samplers have typically used a culture approach or have targeted a specific microorganism in laboratory Cited by: 9. Editorial Reviews. Reviewer: John T. Pierce, MBBS (MD) PhD(Navy Environmental Health Center) Description: This fourth edition of the American Society for Microbiology-sponsored Manual of Environmental Microbiology is written by more than authors and is encyclopedic in scope. Purpose: Underlying the fourth edition is the concept of the minimal functional unit of Price: $
Abstract. Microarrays have unprecedented potential for the high-throughput detection and characterization of uncultivated microorganisms. Several different types of arrays have been developed or adapted for the interrogation of microbial genomes and monitoring microbial population dynamics and/or activity in relation to various microbial processes such as . PCR Results. Only 6 out of 11 (%) H. pylori morphologically and biochemically identified isolates from tap water were found to harbor 16SrRNA gene and of the 3 R.O isolates only one (%) isolate gave positive results for 16SrRNA gene by PCR. Thus leaving out 50% of the conventionally identified isolates as false positive. From the samples negative for H. pylori Cited by:
The sensitivity and specificity of 18S rRNA polymerase chain reaction (PCR) in the detection of fungal aetiology of microbial keratitis was determined in thirty patients with clinical diagnosis of microbial keratitis. Corneal scrapings from patients were used for Gram stain, culture and PCR analysis. PCR was performed with primer pairs targeted to the 18S rRNA by: Environmental Microbiology. The Needle in a Haystack: Detection of Microbes in Source and Drinking Water by Molecular Methods; Taking the Hay Out of the Haystack: Collecting and Processing Water Samples; The Coming Together of the Sciences: Biosensors for the Detection of Waterborne Pathogens Using Antibodies and Gene-Based Recognition Chemistries.
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Gene-Based Detection of Microorganisms in Environmental Samples Using PCR John I. Glass, Elliot J. Lefkowitz, and Gall H.
Cassell Microbiology Department, The University of Alabama at Birmingham Mark Wechser, Theresa B. Taylor, Michael Albin, and Christine Paszko-Kolva Perkin-Elmer, Applied Biosystems Division Monsi C. Roman. Abstract: This chapter examines experimental design considerations for a population-based approach for identifying microorganisms involved in specific in situ functions.
AlthoughCited by: 8. International Conference on Environmental Systems,(27th: Lake Tahoe, Nev.) Investigator(s): Cassell,Gail H; Non-NASA Center: U AL, Birmingham.
Title(s): Gene-based detection of microorganisms in environmental samples using PCR/ John I. Glass. Get this from a library. Gene-based detection of microorganisms in environmental samples using PCR. [John I Glass; United States. National Aeronautics and Space Administration.;].
Culture-based detection of B. pseudomallei cells in environmental soil samples. The single PCR data of the 40 soil samples from this study were compared to the single culture results of the same samples, previously reported as summarized data by Trung and colleagues ().Briefly, B.
pseudomallei cells were detached from the soil matrices of g subsamples by shaking in 50 Cited by: These assays were then assessed by testing well water samples collected in the Québec City region of Canada. Results showed that 97 (%) of the samples tested by culture-based methods and 95 (%), 82 (%), and 98 (%) of samples tested using PCR-based methods contained total coliforms, respectively.
Few representative 16S rRNA gene-based MDMs for pathogen detection in food, environmental and water samples will be discussed in more detail. Wang and co-workers [ 20 ] reported on the development and application of a 16S rRNA gene-based microarray for the detection of food-borne pathogens.
Abstract: This chapter considers the use of molecular methods for direct measures of abundance, diversity and phylogeny of environmental populations of microorganisms. These molecCited by: A considerable number of PCR-based assays have been developed, but they have been applied most often to clinical and environmental samples and more rarely for the detection of.
The objective of this study was to find out the reproducibility and specifity of hipO and ceuE genes based PCR assays for the detection of Campylobacter jejuni isolated from turkey meat samples in a previous study. A total of 44 Campylobacter isolates including 41 C.
jejuni, two C. coli and one C. lari were used in this study. Although all of the C. jejuni isolates were verified by hipO based Cited by: 2. Spiking environmental samples, in particular solid samples, with standard solution followed by immediate extraction, as is the common practice, can lead to an overestimation of the recovery.
As this method enables online detection of the PCR product (Juste and others ), eliminating the need to manipulate PCR products after amplification, the risk of false‐positive results through cross‐contamination between amplification products and subsequent test samples is reduced (Sails and others ; McKillip and Drake ).Cited by: 1.
More specifically, using PCR results as the reference, the performance of traD qPCR was % sensitive and % specific due to identification of four negative PCR samples as positive. Similarly, using IFA results as the reference, traD qPCR showed % sensitivity and % specificity (Table 4) because four samples were mis-identified as Author: Chien-Chung Chao, Tatyana Belinskaya, Zhiwen Zhang, Le Jiang, Wei-Mei Ching.
Many culture-independent molecular microbiology techniques have been developed in the past decades and introduced into oil and gas industry.
These techniques are based on direct extraction of nucleic acids from environmental samples, thus overcoming the difficulties associated with the laboratory cultivation of microorganisms and providing a Author: Xiangyang Zhu, Mohammed A.
Al-Moniee. During the past decades, tremendous advances have been made in the possibilities to study the diversity of microbial communities in the environment. The development of methods to study these communities on the basis of 16S rRNA gene sequences analysis was a first step into the molecular analysis of environmental communities and the study of biodiversity in natural.
The objective of this study was to develop and validate a Taqman real-time PCR assay for the detection of Mycoplasma bovis (M. bovis). Unique primers targeting the highly conserved house-keeping gene (uvrC) were designed and the probe sequence was derived from a previously published microarray study.
There was % agreement in the outcome between our assay Cited by: 5. The field of cultivation-independent microbiology has rapidly advanced over the past few years.
The technological developments briefly described in this chapter have brought us the opportunity to study the enormous complexity of natural microbial communities in more comprehensive and complete terms.
Because the basis of most cultivation-independent approaches in. An endogenous gene target was designed to detect salmonid species DNA in samples. In addition, detection methods using real-time PCR were developed for two GM salmon possessing growth hormone. Manual of Environmental Microbiology (3rd Edition) Detection of Microorganisms in Environmental Freshwaters and Drinking Waters.
View Section, Identifying Microorganisms Involved in Specific in situ Functions: Experimental Design Considerations for rRNA Gene-Based Population Studies and Sequence-Selective PCR. Current technology and its applications in water microbiology, the topics and techniques are equally applicable to all branches of environmental microbiology.
The initial chapters cover the concentration, detection and characterization of microbes in drinking water, other chapters are technology focused and cover topics such as geochips and microarrays and their applications.
Microbial bioremediation serves as an alternative and effective strategy to remove toxic contaminants from a polluted environment. It could be achieved through the interaction of microbes with the toxic contaminants, which leads to immobilization, compartmentalization, and concentration of pollutants rather than their degradation and elimination from the by: 4.Protein-Based Methods for the Detection of GMOs.
Protein-based detection methods are mainly immunological assays, which rely on the use of specific monoclonal or polyclonal antibodies for immunochemical detection (Michelini et al., ).Both monoclonal (highly specific) and polyclonal (highly sensitive) antibodies can be used depending on the amount .The advantages of microfluidic PCR over classical PCR detection methods (i.e.
qualitative PCR, nested PCR, or real-time PCR) can be summarized: (i) small amount of sample is needed for detection of tens of microorganisms, (ii) convenient and easy to implement when thousands of samples are to be tested and (iii) price per sample run is by: 1.